Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral Surgery

The present study was preformed to compare the healing potential of bone marrow derived stem cells (BM-MSCs) versus stem cells derived from human exfoliated teeth (SHED) in treating induced tongue ulcers in a rat animal model. Stem cells may act directly through differentiating into host cells or indirectly through secretion of growth factors and cytokines.The data were based on histopathological and histochemical examination, qRT-PCR gene detection for TGF-Ý1 and histomorphometric analysis. The experiment was conducted on 45 male albino rats. Rats were anesthetized, then tongue ulcers were induced by applying electrocautery pen tip.Two days were left for ulcer establishment. In the 3rd day the rats were randomly distributed into 3 equal groups. The first one was considered as control while the others were considered as experimental groups. The overall results of the present study demonstrated that both BM-MSC and SHED were effective in treatment of induced tongue ulcers. Both showed better healing than the control group. BM-MSCs showed superiority regarding the healed epithelial thickness, amount of mature collagen in early phases of healing and in amount of regenerating muscle fibers. SHED showed more adipocytes and increased innervation; both affect fibroblastic differentiation. Although BM-MSC may be superior to SHED. SHED can be obtained without any invasive procedure. They are obtained from shed deciduous teeth that are considered as a medical waste. TGF-Ý1 level increased by time in all groups. Its higher expression in both BM-MSCs and SHED groups in the early phases of wound healing accelerated wound healing process while its high expression later in the control group results in fibrosis

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