Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Bovine respiratory disease is a major cause of economic losses in dairy beef and feedlot cattle due to increase mortality rate, intensive cost of treatment and prevention in addition to reduction of carcass value. In the present work it was planned to improve the control ofthese infections through acceleration of the field identificationof some of the important pathogens causing these infections.This workwas planned fordevelopment of simple rapid field test. The developed test was designed for identification of two members of the Pasteurellaceae family, Mannheimiahaemolytica (formerly Pasteurellahaemolytica biotype A) and Pasteurellamultocida, these two speciesare frequently associated withBRD.In the present work a fully identified pure culture ofP.multocida and M. hemolytica was obtained and a pathogen specific antigens was prepared.P.multocida and M.hemolytica specific polyclonal antibodies were prepared raised in rabbits and guinea pig.The pathogen specific IgG was purified by caprylic acid method and the titer of this pathogen specific IgG wasdetermined using ELISA.The pathogens specific rabbit polyclonal IgG antibodies were conjugated withcolloidal gold nanoparticles and used for preparation of lateral flow kits for detection of P.multocida and M. hemolytica.The sensitivity,specificity and accuracy of the developed kits were determined using bacteriological examination as astandard gold test It was88.2%,92.4% and 91% respectively For P.multocida and forM.hemolytica80.6%,94.2% and 90% respectively

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