Pharmacological study of the possible therapeutic effect of certain natural and synthetic drugs in experimentally induced osteoporosis in rat / Mohamed Abdelaziz Mahmoud Ibrahim ; Supervised Azza M. Agha , Osama Elshabrawy

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

The current study investigated the antiosteoporotic effects of linseed and fish oils as natural sources of polyunsaturated fatty acids on ovariectomy-induced osteoporosis as compared to alendronate sodium; a nitrogen containing bisphosphonate in this model of experimental osteoporosis. Rats were rendered osteoporotic through bilateral ovariectomy and kept on AIN-93 modified diet for 20 weeks. Linseed oil (90 and 180 mg/kg, p.o), fish oil (90 and180 mg/kg, p.o), and alendronate sodium (3 mg/kg, p.o) were administered to rats for 8 weeks. Efficacy of tested agents was judged based on the estimation of serum calcium (Ca+2), phosphorus, osteocalcin (OCN), bone alkaline phosphatase (BALP), tartrate resistant acid phosphatase (TRAP), tumor necrosis factor alpha (TNF-Ü), as well as histopathological examination of bone sections. Besides, bone mineral densities (BMD) of femur, tibia, and spine were measured by dual x-ray absorptiometry to evaluate the effect of the tested drugs on calcium deposition in bone. Linseed and fish oils hampered serum levels of Ca+2, OCN, BALP, TRAP, and TNF-Ü after both 4 and 8 weeks of its administration to the osteoporotic rats along with an increment in serum phosphorus and femur, spine, and tibia BMD as compared to alendronate sodium. Linseed oil and to a better extent fish oil possess promising anti-osteoporotic effects comparable to alendronate that might be attributed to their contents of polyunsaturated fatty acids. Hence, it can be recommended to use them in osteoporotic patients

Issued also as CD