Micro RNA as a prognostic factor in stage II colorectal cancer patients / Mohamed Elsayed Mostafa Gad Elmola ; Supervised Nasr Mohamed Ali Allahlobi , Abeer Ahmed Bahanassy , Ola Mohamed Reda Khorshid

Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Oncology - Medical

Patients with stage II CRC have a varying survival outcomes, Therefore, it is critical to identify new prognostic and predictive biomarkers that can help to identify more aggressive disease forms among stage II patients and to individualize therapy accordingly. MicroRNAs (miRNAs) are small, noncoding, single stranded RNAs that regulate gene expression post transcriptionally. Present findings have indicated that miRNAs are differently expressed in normal and neoplastic colon tissues and they can distinguish CRC tumors according to histopathologic, prognostic, and predictive characteristics. The aim of this prospective study was to identify the potential prognostic value of five suggested miRNAs, namely; miR 21, miR 498, miR 137, miR - 145 and miR 320, in patients with stage II CRC. Also, this study assessed the concordance between tissue and blood miRNAs. This study included 124 patients with stage II CRC who attended the NCI Medical Oncology clinics during the period from January 2004 to December 2014. All patients included in the study received treatment and were followed - up in the NCI during the last 10 years. The expression levels of the five studied miRNAs were examined by qRT PCR analysis. Additionally, blood samples were drawn from 41 patients recruited in the study in the last 3 years of recruitment to assess the level of miRNAs in blood

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