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Synthesis of novel hybrid steroids with possible cytotoxicity and pro-apoptotic effects on human hepatocellular carcinoma in vitro / Manar Ahmed Ahmed Elhinnawi ; Supervised Rafaat Milad Mohareb , Gamal Abdelmegeed Abdelghany

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Manar Ahmed Ahmed Elhinnawi , 2018Description: 94 P. : charts , facsimiles ; 25cmOther title:
  • تشييد مركبات أسترويدية جديدة و إحتمالية تأثيرها كمضادات لتكاثر الخلايا و محفزات علي الموت المبرمج لخلايا سرطان الكبد خارج جسم الكائن الحي [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechemistry Summary: A series of pregnenolone derivatives were synthesized and evaluated for anti-cancer activity against hepatocellular carcinoma cell line (HepG2). The synthesized steroidal derivatives 3, 4, 5, 6, 7, 8a and 8b were assessed for their cytotoxic activities using MTT (3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay. Apoptotic activity was evaluated using dual acridine orange/ethidium bromide staining method and DNA fragmentation assay. The expression level of the pro-apoptotic genes Bak and Bax as well as anti-apoptotic genes Bcl-xL and Bcl-2 were analyzed using quantitative real time PCR. The results showed that compounds 4 and 6 exhibited cytotoxic activity (IC50s, 36.97 ± 2.18 and 18.46 ± 0.64 oM, respectively), whereas compounds 5 and 7 displayed weak cytotoxic activity (IC50s, 93.87 ± 8.30 oM and 93.48 ± 4.14 oM, respectively). All synthesized pregnenolone derivatives induced apoptosis through DNA fragmentation. Compounds 4 and 6 increased early and late apoptotic cell percentages while compounds 3, 5, 7 and 8b increased either early or late apoptotic cell percentage
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2018.Ma.S (Browse shelf(Opens below)) Not for loan 01010110078872000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2018.Ma.S (Browse shelf(Opens below)) 78872.CD Not for loan 01020110078872000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechemistry

A series of pregnenolone derivatives were synthesized and evaluated for anti-cancer activity against hepatocellular carcinoma cell line (HepG2). The synthesized steroidal derivatives 3, 4, 5, 6, 7, 8a and 8b were assessed for their cytotoxic activities using MTT (3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay. Apoptotic activity was evaluated using dual acridine orange/ethidium bromide staining method and DNA fragmentation assay. The expression level of the pro-apoptotic genes Bak and Bax as well as anti-apoptotic genes Bcl-xL and Bcl-2 were analyzed using quantitative real time PCR. The results showed that compounds 4 and 6 exhibited cytotoxic activity (IC50s, 36.97 ± 2.18 and 18.46 ± 0.64 oM, respectively), whereas compounds 5 and 7 displayed weak cytotoxic activity (IC50s, 93.87 ± 8.30 oM and 93.48 ± 4.14 oM, respectively). All synthesized pregnenolone derivatives induced apoptosis through DNA fragmentation. Compounds 4 and 6 increased early and late apoptotic cell percentages while compounds 3, 5, 7 and 8b increased either early or late apoptotic cell percentage

Issued also as CD

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