Development of immunochromatographic lateral flow test for rapid detection of clostridium perfringens Ü, Ý and f toxins in clinical samples / Moataz Mohamed Magdy Hessin ; Supervised Rafik Tawfik Soliman , Ahmed Samir Mohamed , Yasser Ahmed Abdalla

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Clostridium perfringens toxins is ranked as one of the most common causes of food borne infections and is of major concern as public health problems worldwide for human and animals. The increase in the incidence of foodborne diseases that is often associated with outbreaks, threaten global public health security and raise international concern. Therefore, in the present study a lateral flow immunochromatographic kits have been developed for rapid detection of C. perfringens toxins types, alpha (Ü), beta (Ý) and epsilon (f) in clinical samples. C. perfringens toxins have been prepared, purified and inactivated with 0.2% formalin. Polyclonal antibodies specific to C. perfringens toxins types Ü, Ý and f toxoids have been prepared in rabbits and guinea pigs. The toxin- specific polyclonal antibodies prepared in rabbits were labeled with gold chloride nanoparticles. The prepared toxin specific rabbit and guinea pigs antibodies and goat anti-rabbit antibodies have been utilized in development of lateral flow immunochromatographic test (LFT) that have been evaluated for detection of C. perfringens Ü, Ý and f toxins in clinical samples. The sensitivity, specificity and accuracy of the developed LFT test have been determined by comparison with a commercially available ELISA kits used for detection of these toxins. The prepared LFT was capable to detect C. perfringens Ü, Ý and f toxins in quantities of 2ug/ml, 250ng/ml and 60ng/ml, respectively. 100 poultry suspected fecal samples was examined both with the prepared LFT and ELISA kits for C. perfringens Ü, Ý and f toxins to reveal the validity of developed LFT

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