Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Physiology

Background: Acute kidney injury (AKI) is a kind of acute dysfunction of the kidneys caused by various reasons in a short term, which usually leads to a serious disorder of the body{u2019}s internal environment. Rhabdomyolysis (RM) is considered to be one of the leading causes of AKI. Glycerol induced AKI is well-established animal model of rhabdomyolysis. There is accumulating evidence to suggest the potential benefits of DPP-IV inhibitor is in the setting of AKI. Aim of the work: Our study was designed to study the possible role of dipeptidyl peptidase DPP-IV inhibitor in modulating the pathogenic events related to rhabdomyolysis induced AKI in rats. It also compared between DPP-IV inhibitor and saline as the conventional treatment of AKI concerning their protective effects on AKI when used either separately or in combination and when used for different time intervals of the experiment (24 h and 72 h after injection of glycerol). Methods: Eighty male Albino rats were divided into 5 groups Group I: (control group), Group II; (AKI group), Group III;(AKI+ saline), Group IV;(AKI +sitagliptin 600 mg/kg/day), Group V; (AKI +sitagliptin+ saline), all groups of rats except the control group injected by 50% glycerol (10 ml/kg, i.m.) half dose in each hind limb muscle to induce AKI. All groups were divided into sub groups 8 rats each. Rat tail systolic blood pressure was measured either 24 h or 72 h after glycerol injection. Urine was collected separately for each rat, for 24 h for measurement of urine volume and urinary creatinine levels and GFR calculation. Blood samples were withdrawn retro-orbitally either at 24 h or 72 h after glycerol injection for measuring of serum creatinine, blood urea nitrogen, Na+, K+, creatine phosphokinase (CPK), Glucagon like peptide 1 (GLP1), TNF Ü, interleukin 10. Then 8 rats from each group were sacrificed either at 24 or72hours after injection of glycero

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