Improvement of sugar beet using some new biotechnological techniques / Wessam Mohamed Ali Rslan ; Supervised Mohamed Ibrahim Masr , Naglaa Ahmed Abd Allah , Roba Medhat Ismail

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agronomy

This work was carried out to get synthetic seed and herbicide resistant sugar beet genotypes, using micro-shoot tips encapsulated in 4% (w/v) sodium alginate with 100 mM Ca (NO3) 24H2O as a complex solution. Two solutions were evaluated for their encapsulated vitality efficiency; SA1 solution (MS, 3% sucrose, 4% sodium alginate, 2% sorbitol and 2% mannitol) and SA2 solution (MS, 3% sucrose, 1.3 BAP+4% sodium alginate, 2% sorbitol and 2% mannitol). Synthetic seeds were kept in the dark at 40C to slow down all the tissues growth and stored for different time ranging from 1week to 8 weeks. For getting herbicide resistant plants, agrobacterium carrying the plant vector PISV 2678 was used for transformation of shoot tips of El-Magary and Farida cultivars. PCR and leaf painting test were used to select transgenic plants. Results showed that SA2 was more efficient than SA1 in encapsulated re-growth. Prolonging storage up to 8 weeks reduced significantly germination percentage. Elmagary cultivar had higher transformation efficiency (18%) than farida (12%). Spraying transgenic sugar beet plants with basta had no significant effect on growth traits, but significantly reduced sucrose percentage. Genetic stability of encapsulated plants that was carried out using RAPD PCR analysis showed no significant variability between encapsulated and control plants. Applying this technique can be recommended for developing synthetic seeds as cost effective and time saving method

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