Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Significant variability in weight loss and glycemic control has been observed in obese patients undergoing bariatric surgery. Genetic factors may play a role in the different outcomes. Objectives: The aim of this study is to detect the effect of genetic variants within the FTO gene (rs9939609) and ESR1 gene (rs712221) on weight loss and glycemic control in obese patients 6 months after laparoscopic sleeve gastrectomy. Patients and Methods: Ninety obese patients with BMI {u2265} 35 kg/m2undergoing laparoscopic sleeve gastrectomywere recruited and followed up after 6 months. All were subjected to history taking, clinical examination, routine laboratory investigations and genotyping for FTO gene (rs9939609) and ESR1 gene (rs712221) usingReal Time-PCR (TaqMan probes). Results: FTO (rs9939609) risk genotype AA is found to be associated with more obesity before operation (P-value 0.005) and lead to successful surgery and weight loss (P-value 0.023, OR 0.080, 95%CI 0.009-0.702). Patients with risk allele A had more weight loss and BMI loss after operation compared to patients with non-risk allele T (P-value <0.001). ESR1 (rs712221) risk genotype TT is found to be associated with more weight loss 6 months after surgery when compared to non-risk genotypes TA and AA (P-value 0.001). Patients with risk allele T had more weight loss, BMI loss and HbA1c loss after operation compared to patients with non-risk allele A (P-value <0.001)

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