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Biochemical and molecular studies on the immune system in association with a zoonotic bacterial infection in aquaculture / Marwa Mohamed Mohamed Mohamed Abdalla ; Supervised Fathy Abdalgalel , Iman M. kamel Abumourad

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Marwa Mohamed Mohamed Mohamed Abdalla , 2014Description: 184 P. : charts , facsimiles ; 25cmOther title:
  • دراسات بيوكيميائية وجزيئية على النظام المناعى المصاحب للعدوى البكتيرية الزونوتيكية فى المزارع المائية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: The present study presents the expression of A2M gene in the O. niloticus and albino rats as an immune response to their infection with Zoonotic bacteria. The infection established in fish with IP injection of 0.3 ml of 3X108 CFU{u2215}ml of C. perfringens, and in rats by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after 3- and 6- days post challenge. Liver functions (ALT, AST and ALP) were significantly (P{u2264}0.05) highly elevated. Kidney functions (Urea and uric acid) were not affected but the Cr. showed significant increase in its level (P{u2264}0.05). And the Ca. showed significant decrease in its level (P{u2264}0.05). CRP elevated in rats and O. niloticus. Expression pattern of A2M gene was investigated using a semi- quantitative RT-PCR in liver, spleen and kidney tissues, which showed high expression after the bacterial infection indicating that A2M is immune responder against C. perfringens infection
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Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2014.Ma.B (Browse shelf(Opens below)) Not for loan 01010110066430000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2014.Ma.B (Browse shelf(Opens below)) 66430.CD Not for loan 01020110066430000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry

The present study presents the expression of A2M gene in the O. niloticus and albino rats as an immune response to their infection with Zoonotic bacteria. The infection established in fish with IP injection of 0.3 ml of 3X108 CFU{u2215}ml of C. perfringens, and in rats by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after 3- and 6- days post challenge. Liver functions (ALT, AST and ALP) were significantly (P{u2264}0.05) highly elevated. Kidney functions (Urea and uric acid) were not affected but the Cr. showed significant increase in its level (P{u2264}0.05). And the Ca. showed significant decrease in its level (P{u2264}0.05). CRP elevated in rats and O. niloticus. Expression pattern of A2M gene was investigated using a semi- quantitative RT-PCR in liver, spleen and kidney tissues, which showed high expression after the bacterial infection indicating that A2M is immune responder against C. perfringens infection

Issued also as CD

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