Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Long non coding RNAs (lncRNAs) such as lncRNA-AF085935, lncRNA-uc003wbd, highly upregulated liver cancer (HULC) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) have been involved in pathogenesis of hepatocellular carcinoma (HCC). Single nucleotide polymorphism (SNP) in HULC and MALAT1 has an impact on susceptibility to HCC. However, association between these SNPs and serum lncRNA-AF085935 and lncRNA-uc003wbd levels and their significance in differentiation of HCC from hepatitis B virus (HBV) infected Egyptian patients and the healthy subjects haven{u2019}t been explored yet. In the present study, HULC rs7763881 and MALAT1 rs619586 SNPs were genotyped in 70 HBV-positive HCC,70 HBV patients and 70 healthy controls in Egyptian population and serum levels of lncRNA-AF085935 and lncRNA-uc003wbd of all the participants were investigated by quantitative real-time (qRT) PCR. HULC rs7763881 AC/CC genotype significantly decreased HCC risk. Also, MALAT1 rs619586 AG/GG genotype decreased HCC susceptibility with a borderline significance. Serum lncRNA-AF085935 and lncRNA-uc003wbd levels were higher in HBV-positive HCC and HBV patients compared to controls. ROC analysis distinguished between the three groups and predicted lncRNAs performance in HCC and HBV diagnosis.Serum lncRNA-AF085935 and lncRNA-uc003wbd levels were lower in patients carrying AC/CC genotype of rs7763881 and AG/GG of rs619586 compared with AA genotype. In conclusion, HULC and MALAT1 SNPs are associated with decreased HCC risk in HBV infected Egyptian patients and are correlated with serum lncRNA-AF085935 and lncRNAuc003wbd levels, diagnostic HCC biomarkers

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