Prevalence of mycobacteria other than tuberculosis in clinical isolates from Cairo university hospital in one year duration and species identification using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry / Amira Mohamed Abbas Salama ; Supervised Sahar Mohamed Khairat , Mohamed Ahmed Awad Elbashar, Maha Ali khalaf

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Amira Mohamed Abbas Salama

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TextLanguage: English Publication details: Cairo : Amira Mohamed Abbas Salama , 2021Description: 122 P . : charts , facsmilies ; 25cmOther title:

معدل إنتشار المتفطرات غير السل فى العزلات الإكلينيكية من مستشفى جامعة القاهرة لمدة عام واحد والتعرف على الفصائل باستخدام جهاز قياس الطيف الكتلى بمساعدة الليزر [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: My cobacteria other than tuberculosis (MOTT) are globally recognized as pathogens especially in immune-compromised population. Species-level identification of MOTT is becoming increasingly clinically important. Patients and Methods: A total of 810 samples were involved and the positive culture isolates underwent Identification Test for MOTT identification then species identification by LCD array MycoDirect 1.7 and MALDI-TOF MS. Results: A total of 58 mycobacteria culture positive out of suspected 810 samples which were collected from Cairo University Hospital, Egypt. The prevalence of MOTT was 0.7 % among total samples and 10.3% among positive culture samples MOTT species identification was done by MALDI-TOF MS from strains isolated on Lowenstein Jensen. We performed the recommended protocol of heat inactivation and extraction. Also, Identification was simultaneously done by other techniques as LCD array MycoDirect 1.7, 16S rRNA and WGS for some MOTT isolates as we were hindered by financial issue. Our six MOTT isolates were identified by MALDI TOF MS as two M. fortuitum (33.3%) isolates, one M. abscessus (16.7%) isolate, one M. porcinum (16.7%) isolate, one M. gordonae (16.7%) isolate and one M. bacteremicum (16.7%) isolate. Matching to MALDI TOF MS identification we could perform identification to M. fortuitum by 16 sRNA which was identifiesd as M. neworleansense and it is closely related specied to M. fortuitum groupM.abscessus which was identified by MALDI TOF was also yielded by WGS. Also, LCD array MycoDirect 1.7 results for five strains with the MALDI TOF were concordance but failed to recognize M.bacteremicum

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.07.Ph.D.2021.Am.P (Browse shelf(Opens below))		Not for loan		01010110083778000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.07.Ph.D.2021.Am.P (Browse shelf(Opens below))	83778.CD	Not for loan		0120110083778000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

My cobacteria other than tuberculosis (MOTT) are globally recognized as pathogens especially in immune-compromised population. Species-level identification of MOTT is becoming increasingly clinically important. Patients and Methods: A total of 810 samples were involved and the positive culture isolates underwent Identification Test for MOTT identification then species identification by LCD array MycoDirect 1.7 and MALDI-TOF MS. Results: A total of 58 mycobacteria culture positive out of suspected 810 samples which were collected from Cairo University Hospital, Egypt. The prevalence of MOTT was 0.7 % among total samples and 10.3% among positive culture samples MOTT species identification was done by MALDI-TOF MS from strains isolated on Lowenstein Jensen. We performed the recommended protocol of heat inactivation and extraction. Also, Identification was simultaneously done by other techniques as LCD array MycoDirect 1.7, 16S rRNA and WGS for some MOTT isolates as we were hindered by financial issue. Our six MOTT isolates were identified by MALDI TOF MS as two M. fortuitum (33.3%) isolates, one M. abscessus (16.7%) isolate, one M. porcinum (16.7%) isolate, one M. gordonae (16.7%) isolate and one M. bacteremicum (16.7%) isolate. Matching to MALDI TOF MS identification we could perform identification to M. fortuitum by 16 sRNA which was identifiesd as M. neworleansense and it is closely related specied to M. fortuitum groupM.abscessus which was identified by MALDI TOF was also yielded by WGS. Also, LCD array MycoDirect 1.7 results for five strains with the MALDI TOF were concordance but failed to recognize M.bacteremicum

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