Evaluation of the genetic polymorphism in pre-microRNA-146a (rs2910164) and level of serum tumor necrosis factor alpha in patients with ischemic stroke and their effects on disease prognosis / Shaymaa Mohammed Abdelrahman Elasmer ; Supervised Manal Mohamed Kamal , Hanan Helmy Mohammed Elgendy , Radwa Marawan Abdelhalim

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Inflammation plays a role in the pathogenesis of atherosclerosis and consequently ischemic stroke. Tumor necrosis factor-Ü (TNF-Ü) initiates a cascade of release of cytokines that increases the expression of adhesion molecules on the vascular endothelium. Moreover, single nucleotide polymorphism in Pre-miR-146a may contribute to susceptibility to ischemic stroke by altering mature miR-146a expression levels. Aim of the work: To investigate the association between the pre-miR-146a C> G (r2910164) polymorphism and serum TNF-Ü among group with ischemic stroke and in a group of unrelated volunteers. Subjects &Methods: This was a case control study on 75 cases with ischemic stroke and 75 sex matched control subjects with age range from 57-65 years. Demographic data and computerized tomography confirmation of ischemic stroke were taken from patients files. All samples were subjected to genomic DNA analysis for pre-miR-146a by real time PCR based method and TNF-Ü measurement by enzyme linked immunosobant assay. Results: The frequency of GG genotype within pre-miR-146a (rs2910164) in ischemic stroke cases was significantly different compared to the control subjects with P = 0.017, OR =3.07, 95%CI (1.2-7.7). A highly statistically significant increase in TNF-Ü level in ischemic stroke subjects when compared to the control group; P <0.001

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