header
Local cover image
Local cover image
Image from OpenLibrary

In vitro studies on drought tolerance using gene transfer on tomato / Doaa Hassan Ali Soliman ; Supervised Ahmed Hussein Hanafy Ahmed , Ayman Yehia Amin , Hamdy Abdeaziz Moursy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Doaa Hassan Ali Soliman , 2015Description: 99 P. : facsimiles ; 25cmOther title:
  • دراسات معملية على تحمل الجفاف في الطماطم باستخدام النقل الجيني [Added title page title]
Subject(s): Online resources: Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany Summary: The aim of this study is to investigate best conditions of previously published protocols on Agrobacterium-mediated transformation of tomato, to deliver PtdIns PLC2 gene, which renders the trait of tolerance to drought, to Moneymaker (MM), Super Strain B (SSB) and Rio Grande (RG) tomato cultivars. The obtained results indicated that the efficiency of Agrobacterium-mediated gene transfer in tomato tissues is influenced by many factors; the most important are cultivars, explants type and bacterial optical density. The highest transformation efficiency was 68% with Moneymaker flamingo bill like explant. Whereas the transformation efficiency with Super Strain B and Rio Grande cotyledons were 48% and 40%, respectively. On contrary, the transformation efficiency of Rio Grande and Super Strain B hypocotyls were 20% and 16 %, respectively. The best optical density for cotyledons and hypocotyls of the SSB and RG was 0.05 followed by 0.5 and 1.0. The presence of transgenes and their expression were confirmed by molecular analysis (PCR, RT PCR and Real Time PCR) and histochemical analysis (GUS assay)
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.05.M.Sc.2015.Do.I (Browse shelf(Opens below)) Not for loan 01010110066592000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.05.M.Sc.2015.Do.I (Browse shelf(Opens below)) 66592.CD Not for loan 01020110066592000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany

The aim of this study is to investigate best conditions of previously published protocols on Agrobacterium-mediated transformation of tomato, to deliver PtdIns PLC2 gene, which renders the trait of tolerance to drought, to Moneymaker (MM), Super Strain B (SSB) and Rio Grande (RG) tomato cultivars. The obtained results indicated that the efficiency of Agrobacterium-mediated gene transfer in tomato tissues is influenced by many factors; the most important are cultivars, explants type and bacterial optical density. The highest transformation efficiency was 68% with Moneymaker flamingo bill like explant. Whereas the transformation efficiency with Super Strain B and Rio Grande cotyledons were 48% and 40%, respectively. On contrary, the transformation efficiency of Rio Grande and Super Strain B hypocotyls were 20% and 16 %, respectively. The best optical density for cotyledons and hypocotyls of the SSB and RG was 0.05 followed by 0.5 and 1.0. The presence of transgenes and their expression were confirmed by molecular analysis (PCR, RT PCR and Real Time PCR) and histochemical analysis (GUS assay)

Issued also as CD

There are no comments on this title.

to post a comment.

Click on an image to view it in the image viewer

Local cover image