header
Local cover image
Local cover image
Image from OpenLibrary

mazEF toxin-antitoxin system expression in methicillin resistant and methicillin sensitive staphylococcus aureus and its relation to biofilm-formation / Aya Bakr Elesawi ; Supervised Heba Hamed Arnaout , Yasmine Samy Elkholy , Rania Yahia Shash

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Aya Bakr Elesawi , 2021Description: 105 P. : charts , facsimiles ; 25cmOther title:
  • السم ومضاد السم بين الكريات العنقودية الذهبية المقاومة و الحساسة للميثيسيلين وعلاقته بتكوين غشاء حيوى mazEF مقارنة نواتج النظام الجينى [Added title page title]
Subject(s): Online resources: Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: Background: Staphylococcus aureus is a common cause of many healthcare-associated and community-acquired infections. Methicillin resistance in S. aureus has become prevalent worldwide. Moreover biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. mazEF toxin antitoxin system consists of a stable toxin called mazF which acts as an endoribonuclease and a labile antitoxin called mazE which opposes the toxin action. mazEF genes are located on S. aureus chromosome and can be used as an alternative target in treatment of infections. Objectives: The aim of the present work was to detect mazEF expression in MRSA isolates compared to MSSA isolates as well as determining mazEF system expression in relation to biofilm-formation. Methods: One hundred non-duplicate S. aureus isolates were collected from patients admitted to Kasr Al-Aini University Hospitals and categorized into 50 MRSA and 50 MSSA. All isolates were tested for antimicrobial susceptibility using disk diffusion method and biofilm-formation using tissue culture plate method. Finally, mazEF expression was detected by real time PCR and correlated to both methicillin susceptibility and biofilm-formation. Results: MRSA isolates were shown to be more resistant to antimicrobial agents tested than MSSA isolates. Linezolid and gentamycin showed the best sensitivity results with both MRSA and MSSA. 88% of MRSA and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA and 84% of MSSA isolates were sensitive to gentamycin. Biofilm-formation was statistically significantly higher in MRSA isolates than MSSA isolates (p value = 0.037). Expression of mazEF was statistically significantly higher in MRSA isolates compared to MSSA isolates (p value < 0.001), but no statistically significant correlation was detected between mazEF expression and biofilm-formation
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2021.Ay.M (Browse shelf(Opens below)) Not for loan 01010110083280000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2021.Ay.M (Browse shelf(Opens below)) 83280.CD Not for loan 01020110083280000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Background: Staphylococcus aureus is a common cause of many healthcare-associated and community-acquired infections. Methicillin resistance in S. aureus has become prevalent worldwide. Moreover biofilm-formation makes it more difficult to eradicate bacteria by antibiotics. mazEF toxin antitoxin system consists of a stable toxin called mazF which acts as an endoribonuclease and a labile antitoxin called mazE which opposes the toxin action. mazEF genes are located on S. aureus chromosome and can be used as an alternative target in treatment of infections. Objectives: The aim of the present work was to detect mazEF expression in MRSA isolates compared to MSSA isolates as well as determining mazEF system expression in relation to biofilm-formation. Methods: One hundred non-duplicate S. aureus isolates were collected from patients admitted to Kasr Al-Aini University Hospitals and categorized into 50 MRSA and 50 MSSA. All isolates were tested for antimicrobial susceptibility using disk diffusion method and biofilm-formation using tissue culture plate method. Finally, mazEF expression was detected by real time PCR and correlated to both methicillin susceptibility and biofilm-formation. Results: MRSA isolates were shown to be more resistant to antimicrobial agents tested than MSSA isolates. Linezolid and gentamycin showed the best sensitivity results with both MRSA and MSSA. 88% of MRSA and 96% of MSSA isolates were sensitive to linezolid while 76% of MRSA and 84% of MSSA isolates were sensitive to gentamycin. Biofilm-formation was statistically significantly higher in MRSA isolates than MSSA isolates (p value = 0.037). Expression of mazEF was statistically significantly higher in MRSA isolates compared to MSSA isolates (p value < 0.001), but no statistically significant correlation was detected between mazEF expression and biofilm-formation

Issued also as CD

There are no comments on this title.

to post a comment.

Click on an image to view it in the image viewer

Local cover image