Synthesis and characterization of modified metal based nano materials and their applications /
Mohamed Ahmed Mohamed Abdel Wahab,
Synthesis and characterization of modified metal based nano materials and their applications / التأثير العلاجي للميلاتونين مع / أو الخلايا الجذعية الوسيطة المشتقة من النسيج الدهني على السمية الكبدية الكلوية المستحثة بالدوكسوروبيسين –في جرذان ويستار by Mohamed Ahmed Mohamed Abdel Wahab ; Supervisors Prof. Dr. Mohamed A. Zaye, Dr. Ayman S. Eliwa. - 116 pages : illustrations ; 25 cm. + CD.
Thesis (M.Sc)-Cairo University, 2024.
Bibliography: pages 97-112.
Doxorubicin (DOX), is highly potent against cancer, but it is limited due to its severe side effects, including hepatotoxicity and renal toxicity. This toxicity is caused by oxidative stress, resulting in biochemical, histological, and genetic abnormalities. The antioxidant activity of Melatonin (MEL) and the regenerative properties of Adipose-derived mesenchymal stem cells (ADMSCs) show a promising strategy against DOX-induced toxicity. This study investigated the therapeutic effects of MEL & ADMSCs against DOX-induced toxicity. The research aimed to find whether the antioxidant properties of MEL, the regenerative capabilities, and the anti-inflammatory effect of ADMSCs could synergistically alleviate the adverse effects of DOX. The study included 2 main groups: a negative control group and a DOX group receiving 6 doses of DOX to induce organ toxicity. Subsequently, rats from both groups were subdivided to receive treatments of saline, MEL, ADMSCs, or a combination of them. Rats were euthanized, then blood and tissue samples were collected. The analysis included the evaluation of histopathological changes, oxidative stress biomarkers, gene expression profiles, and DNA damage. DOX induces oxidative stress, inflammation, and genotoxicity in livers and kidneys while reducing antioxidant enzyme activity. MEL and/or ADMSCs treatments effectively mitigate these effects, suggesting the protective mechanisms involved in apoptosis pathways, evidenced by changes in p53 and Bcl-2 expression levels, as well as assessment of double-stranded breaks using the neutral comet assay, and also improvement of the inflammatory markers IL-6 & IL-10 evidenced by the histopathological changes. MEL and ADMSCs counteract the adverse effects caused by DOX, potentially enhancing chemotherapy safety. The synergistic benefits of combination therapy in improving chemotherapy tolerability allow further investigation in preclinical models to optimize treatment strategies and support efficacy before clinical application. الهدف من هذه الأطروحة هو تخليق قاعدة شييف كرا بطة عضيوية صيلبة طويلة )رابط( في بنية .MOF دراسات فيزيائية كيميائية على را بطة قاعدة شيف المصنعة باستخدام FT-IR و XRD و 1HNMR وقييا الطيف الكتلي والتحلييل الحراري. أي لضييييا، تخليق أطر عضييييويية معيدنيية يدييدة، Co-MOF من خلال تفاعل أيونات الكوبلت (II) وقاعدة شيف تحت ظروف التفاعل المائي الحراري التقليدية. تم اسييتخدام العديد من أدوات التوصيييف لإلقاء الضييوء على بنية وسييلو MOFs المصيينعة (Co-MOF) مثل FTIR، حيود الأشييييعة السييييينية للمسييييحوق، قياسييييات التحليل الوزني الحراري (TGA)، تحليل (BET) Brunauer-Emmett-Teller، المجهر الإلكتروني الماسيح نبلا للى ن مع تقنييات قييا .EDX تم التحقيق في MOFs المصيييينعية (Co-MOF) كمحفزات كهربيائيية فعيالية للعديد من التطبيقات البيئية مثل تقسيم الماء والأكسدة الكهربائية للميثانول.
Text in English and abstract in Arabic & English.
Inorganic Chemistry
الكيمياء غير العضوية
Metal-organic framework (MOF) Electrochemical oxidation Water splitting Fuel cell, X-ray diffraction (XRD) scanning electron microscope (SEM) energy dispersive X-ray (EDX)
546
Synthesis and characterization of modified metal based nano materials and their applications / التأثير العلاجي للميلاتونين مع / أو الخلايا الجذعية الوسيطة المشتقة من النسيج الدهني على السمية الكبدية الكلوية المستحثة بالدوكسوروبيسين –في جرذان ويستار by Mohamed Ahmed Mohamed Abdel Wahab ; Supervisors Prof. Dr. Mohamed A. Zaye, Dr. Ayman S. Eliwa. - 116 pages : illustrations ; 25 cm. + CD.
Thesis (M.Sc)-Cairo University, 2024.
Bibliography: pages 97-112.
Doxorubicin (DOX), is highly potent against cancer, but it is limited due to its severe side effects, including hepatotoxicity and renal toxicity. This toxicity is caused by oxidative stress, resulting in biochemical, histological, and genetic abnormalities. The antioxidant activity of Melatonin (MEL) and the regenerative properties of Adipose-derived mesenchymal stem cells (ADMSCs) show a promising strategy against DOX-induced toxicity. This study investigated the therapeutic effects of MEL & ADMSCs against DOX-induced toxicity. The research aimed to find whether the antioxidant properties of MEL, the regenerative capabilities, and the anti-inflammatory effect of ADMSCs could synergistically alleviate the adverse effects of DOX. The study included 2 main groups: a negative control group and a DOX group receiving 6 doses of DOX to induce organ toxicity. Subsequently, rats from both groups were subdivided to receive treatments of saline, MEL, ADMSCs, or a combination of them. Rats were euthanized, then blood and tissue samples were collected. The analysis included the evaluation of histopathological changes, oxidative stress biomarkers, gene expression profiles, and DNA damage. DOX induces oxidative stress, inflammation, and genotoxicity in livers and kidneys while reducing antioxidant enzyme activity. MEL and/or ADMSCs treatments effectively mitigate these effects, suggesting the protective mechanisms involved in apoptosis pathways, evidenced by changes in p53 and Bcl-2 expression levels, as well as assessment of double-stranded breaks using the neutral comet assay, and also improvement of the inflammatory markers IL-6 & IL-10 evidenced by the histopathological changes. MEL and ADMSCs counteract the adverse effects caused by DOX, potentially enhancing chemotherapy safety. The synergistic benefits of combination therapy in improving chemotherapy tolerability allow further investigation in preclinical models to optimize treatment strategies and support efficacy before clinical application. الهدف من هذه الأطروحة هو تخليق قاعدة شييف كرا بطة عضيوية صيلبة طويلة )رابط( في بنية .MOF دراسات فيزيائية كيميائية على را بطة قاعدة شيف المصنعة باستخدام FT-IR و XRD و 1HNMR وقييا الطيف الكتلي والتحلييل الحراري. أي لضييييا، تخليق أطر عضييييويية معيدنيية يدييدة، Co-MOF من خلال تفاعل أيونات الكوبلت (II) وقاعدة شيف تحت ظروف التفاعل المائي الحراري التقليدية. تم اسييتخدام العديد من أدوات التوصيييف لإلقاء الضييوء على بنية وسييلو MOFs المصيينعة (Co-MOF) مثل FTIR، حيود الأشييييعة السييييينية للمسييييحوق، قياسييييات التحليل الوزني الحراري (TGA)، تحليل (BET) Brunauer-Emmett-Teller، المجهر الإلكتروني الماسيح نبلا للى ن مع تقنييات قييا .EDX تم التحقيق في MOFs المصيييينعية (Co-MOF) كمحفزات كهربيائيية فعيالية للعديد من التطبيقات البيئية مثل تقسيم الماء والأكسدة الكهربائية للميثانول.
Text in English and abstract in Arabic & English.
Inorganic Chemistry
الكيمياء غير العضوية
Metal-organic framework (MOF) Electrochemical oxidation Water splitting Fuel cell, X-ray diffraction (XRD) scanning electron microscope (SEM) energy dispersive X-ray (EDX)
546