Microbiological and biochemical studies on the production and stabilization of penicillin acylase /
Marwa Ibrahim Abdelghany Wahba
Microbiological and biochemical studies on the production and stabilization of penicillin acylase / دراسات ميكروبيولوجية و بيوكيميائية لإنتاج و تثبيت إنزيم البنسيلين أسيليز Marwa Ibrahim Abdelghany Wahba ; Supervised Magdy Ali Amin , Ahmed Eldiwany , Magdy Elnashar - Cairo : Marwa Ibrahim Abdelghany Wahba , 2014 - 274 P. : charts ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
The production of microbial penicillin G acylase (PGA) was investigated by six bacterial and two actinomycetal isolates. The PGA production by the chosen bacterial isolate, Arthrobacter viscosus NRRL B-1973, was optimized, appling statistically designed experiments. First, the Plackett-Burman design (PBD) was used to screen the effect of 19 factors, including medium components and culture conditions. Among the nineteen tested factors, only six were proven to be significant. These were; tryptone, arginine, maltose and dextrin, which exerted a significant positive effect on the PGA production, while both MgSO4 and NaCl offered significant negative effects
Arthrobacter viscosus NRRL B-1973 Penicillin G acylase Plackett Burman design
Microbiological and biochemical studies on the production and stabilization of penicillin acylase / دراسات ميكروبيولوجية و بيوكيميائية لإنتاج و تثبيت إنزيم البنسيلين أسيليز Marwa Ibrahim Abdelghany Wahba ; Supervised Magdy Ali Amin , Ahmed Eldiwany , Magdy Elnashar - Cairo : Marwa Ibrahim Abdelghany Wahba , 2014 - 274 P. : charts ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
The production of microbial penicillin G acylase (PGA) was investigated by six bacterial and two actinomycetal isolates. The PGA production by the chosen bacterial isolate, Arthrobacter viscosus NRRL B-1973, was optimized, appling statistically designed experiments. First, the Plackett-Burman design (PBD) was used to screen the effect of 19 factors, including medium components and culture conditions. Among the nineteen tested factors, only six were proven to be significant. These were; tryptone, arginine, maltose and dextrin, which exerted a significant positive effect on the PGA production, while both MgSO4 and NaCl offered significant negative effects
Arthrobacter viscosus NRRL B-1973 Penicillin G acylase Plackett Burman design