Study of In vitro inactivation of rabies virus by hydrogen peroxide /
Asmaa Ahmed Abdelghaffar
Study of In vitro inactivation of rabies virus by hydrogen peroxide / دراسة تثبيط فيروس داء الكلب باستخدام بيروكيد الهدروجين Asmaa Ahmed Abdelghaffar ;Supervised Magdy Ali Amin , Amal Emad Eldin , Abeer A. Elhalim Boseila - Cairo : Asmaa Ahmed Abdelghaffar , 2015 - 105 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
Development of safe and protective vaccines against infectious pathogens remains a challenge. Inactivation of rabies virus is a critical step in the production of vaccines and other research reagents. Beta-propiolactone (ÝPL); the currently used inactivating agent for rabies virus is expensive and proved to be carcinogenic in animals. This study aimed to investigate the ability of hydrogen peroxide (H₂O₂) to irreversibly inactivate rabies virus without affecting its antigenicity and immunogenicity in pursuit of finding safe, effective and inexpensive alternative inactivating agents. H₂O₂ 3% rapidly inactivated a Vero cell adapted fixed rabies virus strain designated as FRV/K within two hours of exposure without affecting antigenicity or immunogenicity. No residual infectious virus was detected and the H₂O₂- inactivated vaccine proved to be safe and effective when compared with the same virus harvest inactivated with the classical inactivating agent (ÝPL). Mice immunized with H₂O₂-inactivated rabies virus produced sufficient level of antibodies and were protected when challenged with lethal CVS virus. These findings reinforce the idea that H₂O₂ can replace ÝPL as inactivating agent for rabies virus to reduce time and cost of inactivation process
Hydrogen Peroxide Inactivation Rabies
Study of In vitro inactivation of rabies virus by hydrogen peroxide / دراسة تثبيط فيروس داء الكلب باستخدام بيروكيد الهدروجين Asmaa Ahmed Abdelghaffar ;Supervised Magdy Ali Amin , Amal Emad Eldin , Abeer A. Elhalim Boseila - Cairo : Asmaa Ahmed Abdelghaffar , 2015 - 105 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
Development of safe and protective vaccines against infectious pathogens remains a challenge. Inactivation of rabies virus is a critical step in the production of vaccines and other research reagents. Beta-propiolactone (ÝPL); the currently used inactivating agent for rabies virus is expensive and proved to be carcinogenic in animals. This study aimed to investigate the ability of hydrogen peroxide (H₂O₂) to irreversibly inactivate rabies virus without affecting its antigenicity and immunogenicity in pursuit of finding safe, effective and inexpensive alternative inactivating agents. H₂O₂ 3% rapidly inactivated a Vero cell adapted fixed rabies virus strain designated as FRV/K within two hours of exposure without affecting antigenicity or immunogenicity. No residual infectious virus was detected and the H₂O₂- inactivated vaccine proved to be safe and effective when compared with the same virus harvest inactivated with the classical inactivating agent (ÝPL). Mice immunized with H₂O₂-inactivated rabies virus produced sufficient level of antibodies and were protected when challenged with lethal CVS virus. These findings reinforce the idea that H₂O₂ can replace ÝPL as inactivating agent for rabies virus to reduce time and cost of inactivation process
Hydrogen Peroxide Inactivation Rabies