Phytochemical and biological studies of soil fungi on hepatic diseases /
Abdelhalim Adel Yousof Elgahamy
Phytochemical and biological studies of soil fungi on hepatic diseases / دراسات فيتوكيميائية و بيولوجية لفطريات التربة علي أمراض الكبد Abdelhalim Adel Yousof Elgahamy ; Supervised Ahlam Mahmoud Elfishawy , Nagwa Elsayed A. Awad - Cairo : Abdelhalim Adel Yousof Elgahamy , 2019 - 203 P. : charts , photographs ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy
This study is concerned with two antagonistic soil fungi trichoderma koningii (T. koningii) and Gliocladium roseum (G. roseum); in order to find out new drugs used in the treatment of liver diseases. The liquid culture filtrates of both fungi were subjected to successive extraction using different polar solvents. The phytochemical investigation of the resulted extracts of the two fungi revealed the presence of glycosidescarbohydrates, steroltriterpenes, tannins and nitrogenous metabolites. Only T. koningii produces coumarins and anthraquinones. GCMS analysis was carried out for the prepared saponifiable and non-saponifiable matters of pet. ether extracts of both fungal filtrates. Both fungi proved promising activity as biological control agents according to the antagonistic activity. The obtained extracts from culture filtrates of both fungi were evaluated against different microbial strains. The ethyl acetate and the dichloromethane extracts from both fungal filtrates exhibited the highest activity while pet. ether and butanol extracts showed the lowest activity. Also, all extracts were evaluated against three different human cancer cell lines (HepG2, HCT116 and MCF7). The ethyl acetate extract of T. koningii and dichoromethane extract of G.roseum were the most active agents against HepG2 with IC50 19 ogml and 18.8 ogml respectively. But in case of HCT116; the ethyl acetate extract of T.koningii and pet. ether of G.roseum were the most active agents with IC50 29.5 ogml and 73.3 ogml respectively. While pet. ether extract of G.roseum and dichoromethane extract of T. koningii were the most active agents against MCF7 with IC50 90.1 ogml and 75.2 ogml respectively. Both of ethyl acetate extract of T.koningii and dichoromethane extract of G.roseum were evaluated in the treatment of thioacetamide (TAA) induced liver toxicity. Only ethyl acetate extract of T.koningii has proved the ability to treat TAA induced liver disease
Antagonistic study Gliocladium roseum Trichoderma koningii
Phytochemical and biological studies of soil fungi on hepatic diseases / دراسات فيتوكيميائية و بيولوجية لفطريات التربة علي أمراض الكبد Abdelhalim Adel Yousof Elgahamy ; Supervised Ahlam Mahmoud Elfishawy , Nagwa Elsayed A. Awad - Cairo : Abdelhalim Adel Yousof Elgahamy , 2019 - 203 P. : charts , photographs ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy
This study is concerned with two antagonistic soil fungi trichoderma koningii (T. koningii) and Gliocladium roseum (G. roseum); in order to find out new drugs used in the treatment of liver diseases. The liquid culture filtrates of both fungi were subjected to successive extraction using different polar solvents. The phytochemical investigation of the resulted extracts of the two fungi revealed the presence of glycosidescarbohydrates, steroltriterpenes, tannins and nitrogenous metabolites. Only T. koningii produces coumarins and anthraquinones. GCMS analysis was carried out for the prepared saponifiable and non-saponifiable matters of pet. ether extracts of both fungal filtrates. Both fungi proved promising activity as biological control agents according to the antagonistic activity. The obtained extracts from culture filtrates of both fungi were evaluated against different microbial strains. The ethyl acetate and the dichloromethane extracts from both fungal filtrates exhibited the highest activity while pet. ether and butanol extracts showed the lowest activity. Also, all extracts were evaluated against three different human cancer cell lines (HepG2, HCT116 and MCF7). The ethyl acetate extract of T. koningii and dichoromethane extract of G.roseum were the most active agents against HepG2 with IC50 19 ogml and 18.8 ogml respectively. But in case of HCT116; the ethyl acetate extract of T.koningii and pet. ether of G.roseum were the most active agents with IC50 29.5 ogml and 73.3 ogml respectively. While pet. ether extract of G.roseum and dichoromethane extract of T. koningii were the most active agents against MCF7 with IC50 90.1 ogml and 75.2 ogml respectively. Both of ethyl acetate extract of T.koningii and dichoromethane extract of G.roseum were evaluated in the treatment of thioacetamide (TAA) induced liver toxicity. Only ethyl acetate extract of T.koningii has proved the ability to treat TAA induced liver disease
Antagonistic study Gliocladium roseum Trichoderma koningii