Cloning, expression and characterization of bacterial L - glutaminase /
Taher Talal Mahrous Ahmed
Cloning, expression and characterization of bacterial L - glutaminase / استنساخ و تعبير و توصيف ل- جلوتامينيز البكتيرى Taher Talal Mahrous Ahmed ; Supervised Zienat Kamel Mohamed Ibrahim , Mohamed G. Farahat - Cairo : Taher Talal Mahrous Ahmed , 2021 - 84 P. : charts , fascimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
L-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by NiNTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml
L-Glutaminase Cloning Screening
Cloning, expression and characterization of bacterial L - glutaminase / استنساخ و تعبير و توصيف ل- جلوتامينيز البكتيرى Taher Talal Mahrous Ahmed ; Supervised Zienat Kamel Mohamed Ibrahim , Mohamed G. Farahat - Cairo : Taher Talal Mahrous Ahmed , 2021 - 84 P. : charts , fascimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
L-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by NiNTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml
L-Glutaminase Cloning Screening