Cloning, expression and characterization of bacterial L - glutaminase / (Record no. 82891)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01860cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032839.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211031s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.05.M.Sc.2021.Ta.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Taher Talal Mahrous Ahmed |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning, expression and characterization of bacterial L - glutaminase / |
| Statement of responsibility, etc. | Taher Talal Mahrous Ahmed ; Supervised Zienat Kamel Mohamed Ibrahim , Mohamed G. Farahat |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | استنساخ و تعبير و توصيف ل- جلوتامينيز البكتيرى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Taher Talal Mahrous Ahmed , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 84 P. : |
| Other physical details | charts , fascimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | L-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by Ni{u2013}NTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | L-Glutaminase |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cloning |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Screening |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed G. Farahat , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zienat Kamel Mohamed Ibrahim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.05.M.Sc.2021.Ta.C | 01010110084596000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.05.M.Sc.2021.Ta.C | 01020110084596000 | 22.09.2023 | CD - Rom | 84596.CD |