Antimicrobial Resistance Pattern And Molecular Characterization Of Virulence Factors Of Avian Pathogenic Escherichia Coli And Human Uropathogenic Escherichia Coli In Egypt / (Record no. 171814)
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| fixed length control field | 06506namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20250508113823.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250430s2024 ua a|||fr|||m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 043 ## - GEOGRAPHIC AREA CODE | |
| Geographic area code | f-ua--- |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 636.089 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 636.089 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.10.10.M.Sc.2024.Ya.A. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Yasser Atef Mohamed, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Antimicrobial Resistance Pattern And Molecular Characterization Of Virulence Factors Of Avian Pathogenic Escherichia Coli And Human Uropathogenic Escherichia Coli In Egypt / |
| Statement of responsibility, etc. | By Yasser Atef Mohamed; Under supervision of Prof. Dr. Mahmoud Dardiri Mohamed, Dr. Zeinab Said Ahmad, Dr. Heba Badr Mahmod. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | أنماط المقاومة للمضادات الحيوية و التوصيف الجزيئى لعوامل ضراوة الايشيريشيا كولاى الممرضة للدواجن وايشيريشيا كولاى الممرضة لمجرى بول الانسان فى مصر / |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 101 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.)-Cairo University, 2024. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 82-101. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Avian pathogenic Escherichia coli (APEC) is a ubiquitous poultry disease affecting the global poultry industry's economics. APEC shares numerous similarities and commonalities in serotypes, phylogroups, virulence genes, disease patterns, in vivo transcriptome of murine urinary infection model and chicken challenge model with human uropathogenic E. coli (UPEC). The research aims to detect the shared virulence factors, antimicrobial resistance (AMR) patterns, and extended-spectrum β-lactamase between APEC and UPEC isolates from poultry and humans in Egypt.<br/>Materials and Methods: Livers, lungs, hearts, and spleens of chicken carcasses were sampled from 35 poultry farms. In addition, a total of 17 human urine samples were collected. Isolation and identification were carried out using conventional methods and biochemical tests. The detection of shared virulence factors of UPEC and APEC by polymerase chain reaction PCR and sequencing. Antimicrobial susceptibility testing according to the Clinical Laboratory Standards Institute Guidelines was measured. Double-disk confirmation test was performed for the extended-spectrum β-lactamase test.<br/>Results: twenty nine isolates were retrieved from 52 chicken organs and human urine samples (55.7%). The resistance rate for all isolates was Amoxicillin/clavulanic acid AMC (100%), AMP (93.1%), CAZ (89.7%), and CTX (82.8%). While CTR was (51.7%), NIT (34.5%), CN (24.1%), and COL (27.6%). Although APEC isolates showed AMC (100%), AMP (100%), CAZ (100%), CTX (94.4%), TE (94.4%), SXT(94.4%), C (100%), DO(88.9%), NA(88.9%), CIP(72.2%), CTR (83.3%), NIT (55.6%), GEN (33.3%), COL (38.9%). Only 15/29 (51.7%) of isolates showed ESBL-positive tests. In the present study, 28/29 (96.5%) E. coli isolates revealed multidrug resistance (MDR). The iss gene (20.69%) was the highest in frequency, other virulence genes papC, and vgrG1 were found lower (6.9% and 3.45% respectively).<br/>Conclusion: APEC multidrug resistance high prevalence to all antibiotics for poultry contributes to therapeutic failure. In addition, it can act as a reservoir of human infection by multidrug-resistant pathogens. Furthermore, the results suggest the zoonotic potential of some APEC strains.<br/> |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | الخلفية والهدف: داء العصيات القولونية الإشريكية القولونية للطيور(APEC) هو مرض للدواجن واسع الانتشار يؤثر على اقتصاديات صناعة الدواجن العالمية. يهدف البحث إلى الكشف عن عوامل الضراوة المشتركة وأنماط مقاومة مضادات الميكروبات (AMR) ومادة البيتا لاكتاماز واسعة النطاق بين معزولات APEC وUPEC من الدواجن والانسان في مصر. <br/>المواد والطرق: أخذ خمس وثلاثين عينة من الكبد والرئتين والقلب والطحال من الدجاج التي يشتبه في إصابتها بأمراض الجهاز التنفسي المزمنة. وبالإضافة إلى ذلك، جمع سبعةعشر عينة بول بشرية تحتوي على صديد. إجري العزل والتعرف باستخدام الطرق التقليدية والاختبارات البيوكيميائية. و تقييم الكشف عن عوامل الضراوة المشتركة UPEC وAPEC بواسطة تفاعل البلمرة المتسلسل PCR وعمل اختبار تسلسل النيوكلينيدات.. وقيس اختبار الحساسية للمضادات الحيوية. و إجري اختبار بيتا لاكتميز ممتد الطيف ESBL. <br/>النتائج: استخرج تسعة وعشرين عزلة من اثنين و خمسين عينة من أعضاء دجاج وبول بشري. وكشف عن مقاومة المضادات الحيوية باختبار الحساسية.<br/>AMC (100%)، AMP (93.1%)، (89.7%)CAZ ، (82.8%)CTX. TE (65.5%)، (62.1%),SXT(65.5%)،.DO(58.6%) NA(55.2%)فقط 15/29 (51.7٪) من العزلات أظهرت نتيجة إيجابية لاختبار ESBL. في الدراسة الحالية، أظهرت 28/29 (96.5%) معزولات مقاومة للأدوية المتعددة (MDR). كان جين iss ( 20.69%) هو الأعلى في التكرار، بينما وجدت جينات الضراوة الأخرى papC وvgrG1 أقل (6.9% و3.45% على التوالي). <br/> |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issued also as CD |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Veterinary medicine |
| Source of heading or term | qrmak |
| 653 #0 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Avian pathogenic E. coli |
| -- | colibacillosis, poultry |
| -- | human uropathogenic E. coli |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Dardiri Mohamed |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zeinab Said Ahmad |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heba Badr Mahmod |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2024 |
| Supervisory body | Mahmoud Dardiri Mohamed |
| -- | Zeinab Said Ahmad |
| -- | Heba Badr Mahmod |
| Universities | Cairo University |
| Faculties | Faculty of Veterinary Medicine |
| Department | Department of Microbiology |
| Discussion body | Nagwa Sayed Sayed |
| -- | Ahmed Orabi Hassan |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Eman El gebaly |
| Reviser Names | Eman Ghareb |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 30.04.2025 | 91084 | Cai01.10.10.M.Sc.2024.Ya.A. | 01010110091084000 | 30.04.2025 | 30.04.2025 | Thesis |