Thesis (M.Sc)-Cairo University, 2025.

Bibliography: pages 188-257.

Allolobophora caliginosa has been widely utilized in the pharmaceutical industry because it has many therapeutic properties that attributed to its coelomic fluid. Hence, the current study was intended to identify the bioactive constituents and the biological activities of Allolobophora caliginosa coelomic fluid (ACCF) and its the possible antitumor effect against Ehrlich ascites carcinoma (EAC) in female Swiss albino mice. The effectiveness of ACCF was compared with fluorouracil (Flu) as a standard chemotherapy drug. Various active phenolic and flavonoid constituents and a considerable amount of essential and non-essential amino acids were found in ACCF. The current study clarified that ACCF exhibited dose-dependent radical scavenging and potent anti-inflammatory activities. Furthermore, the proliferation of HepG-2 could be inhibited by ACCF with the IC50 value of 145.99 μg/ml. The present findings revealed that ACCF showed amazing peripherally and centrally analgesic and antipyretic potencies. ACCF has various secondary metabolites that were likely to act synergistically to produce the observed biological activities. Besides, oral administration of ACCF for 9 days caused a significant amelioration in all the hematological parameters relative to the untreated EAC group. Markedly, ACCF administration significantly enhanced hepatic function by the reduction in liver enzyme activities such as ALT, AST, GGT, and lactate as well as the increase in total protein and albumin content. As well as, ACCF treatment improved renal function as indicated by the reduction in serum creatinine, urea and uric acid. Concerning lipid metabolism, ACCF treatment caused a marked enhancement in lipid metabolism, as indicated by a significant decrease in serum total cholesterol and triglycerides levels. Moreover, ACCF treatment could hinder the hepatic and renal oxidative stress induced by EAC via a noticeable increase in both hepatic and renal GSH content and antioxidant enzyme activities (SOD, CAT, and GST) besides reduction in MDA and NO formation. Administration of ACCF repairs, to some extent, the abnormal architecture of hepatic tissue induced by EAC. Finally, ACCF could be used as a prospective anti-tumor and hepato-renal protective alternative remedy.

تهدف الدراسة الحالية إلي تقييم الفعالية المضادة للأورام للسائل الجوفي للألولوبوفرا كاليجينوزا ضد سرطان إيرلش الإستسقائي من خلال التعرف علي المركبات البيولوجية النشطه له. و أوضحت الدراسة الحالية العديد من الأنشطة البيوليوجية المختلفة للسائل الجوفي للألولوبوفرا كاليجينوزا مثل النشاط المضادة للأكسدة, نشاطًا قويًا مضادًا للالتهابات, نشاطاً فعالاً ضد سرطان الكبد الوبائي-2. كما لدية القدرة علي التخفيف من حدة الألم و إنخفاض درجة حرارة الجسم. كما أوضحت الدراسة الحالية أن سرطان إيرلش الإستسقائي تسبب في أضرار غير المرغوب فيها لمعظم القياسات البيوكيميائية. كما وجد تغير باثولوجي في كبد الفئران المصابة بسرطان إيرلش الإستسقائي. بينما أدت المعالجة بالسائل الجوفي للألولوبوفرا كاليجينوزلمدة 9 أيام إلي تثبيط نشاط الخلايا السرطانيه . كما أدت إلي تحسن لمعظم القياسات البيوكيميائية و التركيب النسيجي للكبد إلي مستوي يقترب من التركيب الطبيعي. و هذا وجد علي التوافق مع نتائج الفلورويورسيل كدواء للسرطان.

Issues also as CD.

Text in English and abstract in Arabic & English.