Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. / Rehab Ali Mohammed Mohammed Elhelw ; Supervised Mahmoud Essam Hatem Ahmed , Mona Ibrahim Elnbaawy , Ahmed Abdelghani Elsanousi

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Rehab Ali Mohammed Mohammed Elhelw

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TextLanguage: English Publication details: Cairo : Rehab Ali Mohammed Mohammed Elhelw , 2014Description: 184 P. : facsimiles ; 25cmOther title:

إنتاج مجموعات إليزا علي أساس الأجسام المضادة أحادية الفسيلة لليبتوسبيرا الممرضة [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: A panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freund{u2019}s adjuvant. Booster doses with and without incomplete freund{u2019}s adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained

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Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.Ph.D.2014.Re.P (Browse shelf(Opens below))		Not for loan	01010110063359000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.Ph.D.2014.Re.P (Browse shelf(Opens below))	63359.CD	Not for loan	01020110063359000

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Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

A panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freund{u2019}s adjuvant. Booster doses with and without incomplete freund{u2019}s adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained

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جامعة القاهرة

المكتبة المركزية الجديدة

مكتبة جامعة القاهرة الأهلية

Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. / Rehab Ali Mohammed Mohammed Elhelw ; Supervised Mahmoud Essam Hatem Ahmed , Mona Ibrahim Elnbaawy , Ahmed Abdelghani Elsanousi

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