Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

The study deals with possible antidiabetic action of phosphodiesterase inhibitors in rats. It is composed of in vivo and in vitro studies. In the in vivo study, diabetes was induced by injecting a single dose of streptozotocin (50 mg/kg) then 2 drugs; sildenafil (Sild), vinpocetine (Vinp) were used and gliclazide (Glicl) was used as a reference standard then drugs were administered daily over 2 weeks. The chosen doses were 10 mg/kg for Glicl, 5, 10, 20 mg/kg for Sild, 10, 20, 40 mg / kg for Vinp then glucose, insulin and C - peptide levels were estimated after 2 hours of first dose, 1 week and 2 weeks of drug administration. Also Liver glycogen was measured after 2 weeks. In In vitro study, effect of Vinp, Sild, Glicl was studied individually in concentrations (10, 20, 40, 80 æmol/l) or in combinations for 1 hour on Ý - islets isolated by collagenase digestion technique in glucose (3 or 16.7 mmol/l) and released insulin and cGMP levels were estimated in incubation medium

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