Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Rapid detection of pathogens in food becomes a critical and important demand for human safety, since most foodborne illnesses and deaths are caused by pathogenic bacteria. So application of rapid, sensitive method to detect foodborne pathogen is essential in controlling food safety. In this study, a two multiplex polymerase chain reaction (mPCR) technique for the simultaneous detection of some foodborne pathogens (Salmonella ,S. aureus , Bacillus cereus , Listeria monocytogenes, E. coli and Campylobacter spp.) was done in culture broth and artificial food matrix. Pathogen-specific DNA sequences in the invA, clfA, groEL, 16S rRNA, phoA and 23S rRNA genes were used as targets to design primers for the identification of Salmonella, S. aureus, Bacillus cereus, Listeria monocytogenes, E. coli and Campylobacter spp. respectively. The detection of sensitivity in this assay was 10 CFU/ml of each pathogen in a culture broth and artificially inoculated samples after enrichment for 24 h. The mPCR assay proposed here can gain results within 24 h and correspond to the results obtained by the classical cultivation based on ISO methods, which will be valuable for food safety investigations

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