Thesis ( Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Bibliography: pages 115-137.

Background: Colorectal cancer (CRC) is a complex disease that develops as a consequence of both genetic and environmental risk factors. Diet, smoking and drinking habits are among environmental factors frequently associated with CRC risk. Aim of work:The present study aims to conduct a clinical biochemical study that aids in the investigation of some non-coding RNA expressions and polymorphisms (including long non-coding RNAs and miRNAs) namely, Plasmacytoma Variant Translocation-1 (PVT-1) and miR-186 in an attempt to provide new noninvasive diagnostic biomarkers for CRC of the Egyptian patients. Patients and methods: Eighty CRC studies and thirty healthy controls were included. Laboratory and pathological investigations were assessed. Serum miR-186 and long non-coding PVT-1 was measured as well as genotype for PVT-1 rs13255292. Results: The CRC patients had a mean age of 50.91±12.0. The mean serum miR-186 level in CRC patients (1.38±0.21) was significantly higher than in the control (0.93±0.1) (p=0.0001). The PVT-1 level in CRC patients was (5.91±0.25) significantly higher than control (1.1±0.2) (p=0.001). The PVT-1/ rs13255292 genotype CT was significantly higher compared to the control group. The T allele was highly significant with a p-value of 0.008 in the CRC patient group with respect to the control. Conclusion: miR-186, long noncoding PVT-1 as well as PVT-1 the T allele may be considered as genetic markers for CRC susceptibility

Issued also as CD

Text in English and abstract in Arabic & English.