Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Internal Medicine

Background: Distinguishing self-limited acute ITP from chronic ITP identify potential targets for therapeutic interventions in the group at risk for chronic ITP. Overproduction free radical in the absence of adequate antioxidant defense may cause irreversible changes to biomolecules and the generation of neo-antigenic determinants. This may ultimately contribute to epitope spreading and the abrogation of self-tolerance. Vanin-1 (VNN1) has been proven to be an oxidative stress sensor, which regulates endogenous glutathione levels. Objectives: to find an association between overexpressed VNN1 in blood and progression to chronic ITP. Method: The study was carried out on 40 cases and 10 healthy controls patients were grouped into: Group A: 30 patients with acute ITP Group B: 10 patients with chronic ITP. All patients were subjected to Vanin1 gene level by PCR technique. Results: Vanin1 gene was higher in patients with ITP than control with statistically significant values, (P-value< 0.001) vanin1 gene was higher in acute than chronic patients but the difference was statistically insignificant, (P value >0.05) .correlation between vanin1 gene and hemoglobin, leucocytic count and platelet count before treatment in group B (chronic ITP)and groups A (responder and non-responders) were statistically insignificant

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