MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02511cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
190511s2018 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.11.07.M.Sc.2018.Ha.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hadeel Khaled Mohamed Abdelmoneim |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Expansion of anti-Schistosoma mansoni secretory hybridomas in chemically defined serum free culture as a reliable source for monoclonal antibodies / |
| Statement of responsibility, etc. |
Hadeel Khaled Mohamed Abdelmoneim ; Supervised Shereen Mahmoud Kamel , Kesmat Mohamed Maher , Engy Mohamed Elkhateeb |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
التوسع في انتاج خلايا الورم الهجين المنتجة لمضادات البلهارسيا المانسوني في وسط خالي من المصل لانتاج الاجسام المضادة احادية المصدر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Hadeel Khaled Mohamed Abdelmoneim , |
| Date of publication, distribution, etc. |
2018 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
154 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Introduction: The cultivation of hybridoma cells secreting monoclonal antibodies (MAbs) involves the use of media supplemented with fetal bovine serum (FBS). The serum provides essential nutrients for cell metabolism, growth, and differentiation. However its use is increasingly criticized for scientific, technical, and ethical reasons.This lead to widespread support of cultivation the hybridoma cells in serum free conditions. Methodology: In this work, we studied the effect of the serum supplemented, serum free and chemically defined media on the hybridoma cells{u2019} growth characteristics and monoclonal antibody productivity. To protect the hybridoma cells, cryopreservation and revival under serum free conditions was assessed. Bambanker media was studied for cryopreservation of the cells at -80{u00B0}C instead of liquid nitrogen. Lab-scale production of MAbs and purification was done to diagnose circulation Schistosoma mansoni soluble egg antigen in patient serum by sandwich ELISA |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Hybridoma |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Monoclonal antibody |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Serum-free |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Engy Mohamed Elkhateeb , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kesmat Mohamed Maher , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Shereen Mahmoud Kamel , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Shimaa |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
