header
Image from OpenLibrary

Expansion of anti-Schistosoma mansoni secretory hybridomas in chemically defined serum free culture as a reliable source for monoclonal antibodies / Hadeel Khaled Mohamed Abdelmoneim ; Supervised Shereen Mahmoud Kamel , Kesmat Mohamed Maher , Engy Mohamed Elkhateeb

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hadeel Khaled Mohamed Abdelmoneim , 2018Description: 154 P. : charts , facsimiles ; 25cmOther title:
  • التوسع في انتاج خلايا الورم الهجين المنتجة لمضادات البلهارسيا المانسوني في وسط خالي من المصل لانتاج الاجسام المضادة احادية المصدر [Added title page title]
Subject(s): Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Introduction: The cultivation of hybridoma cells secreting monoclonal antibodies (MAbs) involves the use of media supplemented with fetal bovine serum (FBS). The serum provides essential nutrients for cell metabolism, growth, and differentiation. However its use is increasingly criticized for scientific, technical, and ethical reasons.This lead to widespread support of cultivation the hybridoma cells in serum free conditions. Methodology: In this work, we studied the effect of the serum supplemented, serum free and chemically defined media on the hybridoma cells{u2019} growth characteristics and monoclonal antibody productivity. To protect the hybridoma cells, cryopreservation and revival under serum free conditions was assessed. Bambanker media was studied for cryopreservation of the cells at -80{u00B0}C instead of liquid nitrogen. Lab-scale production of MAbs and purification was done to diagnose circulation Schistosoma mansoni soluble egg antigen in patient serum by sandwich ELISA
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2018.Ha.E (Browse shelf(Opens below)) Not for loan 01010110078110000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2018.Ha.E (Browse shelf(Opens below)) 78110.CD Not for loan 01020110078110000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Introduction: The cultivation of hybridoma cells secreting monoclonal antibodies (MAbs) involves the use of media supplemented with fetal bovine serum (FBS). The serum provides essential nutrients for cell metabolism, growth, and differentiation. However its use is increasingly criticized for scientific, technical, and ethical reasons.This lead to widespread support of cultivation the hybridoma cells in serum free conditions. Methodology: In this work, we studied the effect of the serum supplemented, serum free and chemically defined media on the hybridoma cells{u2019} growth characteristics and monoclonal antibody productivity. To protect the hybridoma cells, cryopreservation and revival under serum free conditions was assessed. Bambanker media was studied for cryopreservation of the cells at -80{u00B0}C instead of liquid nitrogen. Lab-scale production of MAbs and purification was done to diagnose circulation Schistosoma mansoni soluble egg antigen in patient serum by sandwich ELISA

Issued also as CD

There are no comments on this title.

to post a comment.