Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

This work had been carried out to study converting drug (Tamoxifen), some nutrients (yeast) and bioactive compounds (Silymarin and Isoflavone) to nanoparticles and study their different effects in nano and normal scale on gene expression. Encourage the use of nanotechnology to limit the development and spread of breast cancer and the inhibitory effect of them on development of toxic material of mammary tumor for 180 days against with a single oral dose of (25 mg/kg) 7,12-dimethylbenz[a]anthracene (DMBA) in female rats. The body weight and food consumption will be examined. Upon the completion of the study, the histopathological studies on the breast tissues will be performed. The therapeutic and protective effects of these compounds in nano and normal scale will be evaluated using the appropriate parameters to determine the level of several chemicals related to oxidative damage (8-OHdG, Lipid Peroxide, TAC), Apoptosis, Plasma Estrogen and ErbB-2 in the blood samples. Also, the gene expression of ErbB-2 will be investigated in the blood samples. Finally, all the experimental results evaluated, tabulated and statistically analyzed. In conclusion, Breast cancer is highly curable if diagnosed with nutrients or medications whether in their normal or nano particles form at an early stage

Issued also as CD