Microbiological and biochemical studies on the production and stabilization of penicillin acylase / Marwa Ibrahim Abdelghany Wahba ; Supervised Magdy Ali Amin , Ahmed Eldiwany , Magdy Elnashar
Material type:
- دراسات ميكروبيولوجية و بيوكيميائية لإنتاج و تثبيت إنزيم البنسيلين أسيليز [Added title page title]
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.08.06.M.Sc.2014.Ma.M (Browse shelf(Opens below)) | Not for loan | 01010110063363000 | ||
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.08.06.M.Sc.2014.Ma.M (Browse shelf(Opens below)) | 63363.CD | Not for loan | 01020110063363000 |
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Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
The production of microbial penicillin G acylase (PGA) was investigated by six bacterial and two actinomycetal isolates. The PGA production by the chosen bacterial isolate, Arthrobacter viscosus NRRL B-1973, was optimized, appling statistically designed experiments. First, the Plackett-Burman design (PBD) was used to screen the effect of 19 factors, including medium components and culture conditions. Among the nineteen tested factors, only six were proven to be significant. These were; tryptone, arginine, maltose and dextrin, which exerted a significant positive effect on the PGA production, while both MgSO4 and NaCl offered significant negative effects
Issued also as CD
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