Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

This study is concerned with two antagonistic soil fungi trichoderma koningii (T. koningii) and Gliocladium roseum (G. roseum); in order to find out new drugs used in the treatment of liver diseases. The liquid culture filtrates of both fungi were subjected to successive extraction using different polar solvents. The phytochemical investigation of the resulted extracts of the two fungi revealed the presence of glycosides{u2215}carbohydrates, sterol{u2215}triterpenes, tannins and nitrogenous metabolites. Only T. koningii produces coumarins and anthraquinones. GC{u2215}MS analysis was carried out for the prepared saponifiable and non-saponifiable matters of pet. ether extracts of both fungal filtrates. Both fungi proved promising activity as biological control agents according to the antagonistic activity. The obtained extracts from culture filtrates of both fungi were evaluated against different microbial strains. The ethyl acetate and the dichloromethane extracts from both fungal filtrates exhibited the highest activity while pet. ether and butanol extracts showed the lowest activity. Also, all extracts were evaluated against three different human cancer cell lines (HepG2, HCT116 and MCF7). The ethyl acetate extract of T. koningii and dichoromethane extract of G.roseum were the most active agents against HepG2 with IC50 19 og{u2215}ml and 18.8 og{u2215}ml respectively. But in case of HCT116; the ethyl acetate extract of T.koningii and pet. ether of G.roseum were the most active agents with IC50 29.5 og{u2215}ml and 73.3 og{u2215}ml respectively. While pet. ether extract of G.roseum and dichoromethane extract of T. koningii were the most active agents against MCF7 with IC50 90.1 og{u2215}ml and 75.2 og{u2215}ml respectively. Both of ethyl acetate extract of T.koningii and dichoromethane extract of G.roseum were evaluated in the treatment of thioacetamide (TAA) induced liver toxicity. Only ethyl acetate extract of T.koningii has proved the ability to treat TAA induced liver disease

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