Cloning, expression and characterization of bacterial L - glutaminase / Taher Talal Mahrous Ahmed ; Supervised Zienat Kamel Mohamed Ibrahim , Mohamed G. Farahat
Material type:
TextLanguage: English Publication details: Cairo : Taher Talal Mahrous Ahmed , 2021Description: 84 P. : charts , fascimiles ; 25cmOther title: - استنساخ و تعبير و توصيف ل- جلوتامينيز البكتيرى [Added title page title]
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Thesis
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.12.05.M.Sc.2021.Ta.C (Browse shelf(Opens below)) | Not for loan | 01010110084596000 | ||
CD - Rom
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.12.05.M.Sc.2021.Ta.C (Browse shelf(Opens below)) | 84596.CD | Not for loan | 01020110084596000 |
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
L-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by Ni{u2013}NTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml
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